熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
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熱門關(guān)鍵詞： ELISA試劑盒 - 大鼠ELISA試劑盒- 小鼠ELISA試劑盒
全國統(tǒng)一客服熱線：
0755-28715175
深圳子科生物優(yōu)勢代理美國R&DELISA系列產(chǎn)品試劑盒，價格優(yōu)勢，貨期短，歡迎來電咨詢0755-33164177！
貨號：MKM100
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
The recovery of TIM-1 spiked into various matrices was evaluated.
Mouse TIM-1/KIM-1/HAVCR Quantikine ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Urine (10 uL)
Sensitivity
2.18 pg/mL
Assay Range
7.8 - 500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Specificity
Natural and recombinant mouse TIM-1. The antibody components of this kit were raised against the short splice form of mouse TIM-1 (isoform 2) which has a 23 amino acid deletion following Pro182. This immunoassay detects both the short and long forms of mouse TIM-1.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse TIM-1 Immunoassay is a 3.5 hour solid phase ELISA designed to measure mouse TIM-1 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse TIM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse TIM-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TIM-1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean
24.2
61.8
207
23.2
60
190
Standard Deviation
0.8
1.6
6.8
2.5
4.2
13.2
CV%
3.3
2.6
3.3
10.8
7
6.9
Recovery
Sample Type
Average % Recovery
Range %
Cell Culture Media (n=4)
100
91-106
EDTA Plasma (n=4)
89
88-91
Heparin Plasma (n=4)
87
86-89
Serum (n=4)
95
93-97
Urine (n=4)
102
94-115
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse TIM-1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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