Human IL-1ra/IL-1F3 Quantikine ELISA Kit
Human IL-1ra/IL-1F3 Quantikine ELISA Kit
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Human IL-1ra/IL-1F3 Quantikine ELISA Kit Summary
貨號：DRA00B
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-1ra
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1ra showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
66.9
|
607
|
1627
|
82.5
|
646
|
1663
|
|
Standard Deviation
|
4.88
|
30.2
|
60
|
9.04
|
51.4
|
111
|
|
CV%
|
7.3
|
5
|
3.7
|
11
|
8
|
6.7
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
40
|
40
|
40
|
|
Mean
|
64.9
|
538
|
1343
|
56.2
|
469
|
1243
|
|
Standard Deviation
|
3.67
|
22.1
|
55.7
|
5.79
|
31.9
|
70.4
|
|
CV%
|
5.6
|
4.1
|
4.1
|
10.3
|
6.8
|
5.7
|
Recovery
The recovery of IL-1ra spiked to three levels throughout the range of the assay in various matrices was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Cell Culture Supernates (n=4)
|
106
|
96-115
|
|
EDTA Plasma (n=4)
|
86
|
66-102
|
|
Heparin Plasma (n=4)
|
93
|
82-106
|
|
Serum (n=4)
|
90
|
81-99
|
Linearity
To assess the linearity of the assay, samples containing high concentrations of IL-1ra were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
