Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit
Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit
Mouse/Rat/Porcine/Canine TGF-beta 1 Quantikine ELISA Kit Summary
Catalog Size / Price
MB100B 1 Kit / Please Inquire
SMB100B 1 Pack (6 Plates) / Please Inquire
PMB100B 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (40 uL), EDTA Plasma (40 uL), Heparin Plasma (40 uL)
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant TGF-beta 1. This kit also recognizes human TGF-beta 1.2.
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
Interference observed with 1 or more available related molecules.
Product Summary
The Quantikine Mouse/Rat/Porcine/Canine TGF-beta 1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure TGF-beta 1 in acid activated cell culture supernates, serum, and plasma. It contains recombinant TGF-beta 1 expressed by CHO cells and has been shown to quantitate the recombinant factor accurately. Results obtained using natural TGF-beta 1 showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit will provide accurate quantitation for both recombinant and natural TGF-beta 1.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Serum, EDTA Plasma, Heparin Plasma
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
28
|
37
|
30
|
|
Mean
|
78
|
180
|
695
|
79
|
185
|
730
|
|
Standard Deviation
|
2.3
|
3.4
|
17.3
|
7.2
|
17.2
|
46.8
|
|
CV%
|
2.9
|
1.9
|
2.5
|
9.1
|
9.3
|
6.4
|
Cell Culture Supernates
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
22
|
21
|
22
|
|
Mean
|
88
|
198
|
816
|
97
|
209
|
826
|
|
Standard Deviation
|
3
|
4.9
|
19.6
|
8.1
|
17.3
|
47
|
|
CV%
|
3.4
|
2.5
|
2.4
|
8.4
|
8.3
|
5.7
|
Recovery
The recovery of TGF-beta 1 spiked to levels throughout the range of the assay followed by activation was evaluated.
|
Sample Type
|
Average % Recovery
|
Range %
|
|
Mouse Cell Culture Supernates (n=4)
|
105
|
83-114
|
|
Porcine Cell Culture Supernates (n=2)
|
109
|
102-118
|
|
Rat Cell Culture Supernates (n=3)
|
112
|
93-125
|
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