Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit
Mouse IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary
Catalog Size / Price
MLB00C 1 Kit (2 Plates) / Please Inquire
SMLB00C 1 Pack (6 Plates) / Please Inquire
PMLB00C 1 Pack (50 Plates) / Please Inquire
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Tissue Lysates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Assay Range
12.5 - 800 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Product Summary
The Quantikine® Mouse IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-1 beta in cell culture supernates, tissue lysates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse IL-1 beta showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-1 beta.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
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|
Intra-Assay Precision
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Inter-Assay Precision
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|
Sample
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1
|
2
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3
|
1
|
2
|
3
|
|
n
|
20
|
20
|
20
|
20
|
20
|
20
|
|
Mean
|
33.5
|
92.9
|
386
|
34.6
|
93.5
|
423
|
|
Standard Deviation
|
2.5
|
4.3
|
11.7
|
2.9
|
6.2
|
23.9
|
|
CV%
|
7.5
|
4.6
|
3
|
8.4
|
6.6
|
5.7
|
Recovery
The recovery of mouse IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.
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Sample Type
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Average % Recovery
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Range %
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|
Cell Culture Supernates (n=4)
|
106
|
95-119
|
|
EDTA Plasma (n=4)
|
108
|
101-117
|
|
Heparin Plasma (n=4)
|
97
|
87-114
|
|
Serum (n=4)
|
105
|
98-113
|
|
Tissue Lysates (n=4)
|
115
|
110-120
|
